P002 Exclusive Enteral Nutrition, the gut microbiome and corticosteroid response in patients with acute severe colitis

Abstract Background Supplementation with exclusive enteral nutrition (EEN) in addition to standard of care intravenous corticosteroids (SOC) augments the steroid response patients acute severe ulcerative colitis (ASUC). EEN is known alter gut microbial composition. The present study identifies changes microbiota driven by ASUC receiving therapy and examines correlations between EEN-associated bacteria corticosteroid therapy. Methods Stool samples were collected at baseline (Day0) post-therapy (Day 7) from 44 who received either SOC (n=24) or supplemented (n=20) for a period 7 days, 0) (Day-7). Microbiome characterization was carried out using 16S rRNA gene sequencing followed data processing QIIME2 R packages analyze diversity differentially abundant taxa. Results Seven-day supplementation resulted enhanced proportions Methylobacterium, Sphingomonas, Limosilactobacillus, Megamonas, Thermus, Viellonella, reduction Ruminococcus gnavus, Gemmiger, Pseudomonas, Enterococcus. EEN-mediated enhancement specific taxa correlated positively patients’ day-7 serum albumin levels negatively day-3 faecal calprotectin (FCal) (Fig.1). Gut composition responded EEN-augmented showed an abundance Coprococcus, Oribacterium, Sediminibacterium, Acidibacter, Sutterella, R. Collinsella, Dorea, Morganella, when compared non-responders (Fig.2). Baseline microbiome signature predicts potential both EEN-supplemented (Fig.3). Conclusion Augmentation clinical EEN-conjugated accompanied ASUC.